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AP0507: Principles of Cellular and Biomolecular Analysis Report, NU, Singapore TNF-alpha ELISA Practical Report Aim of this experiment: culture U937 cells, differentiate them with PMA
University Northumbria University (NU)
Subject AP0507: Principles of Cellular and Biomolecular Analysis
Posted on: 25th Sep 2023

AP0507: Principles of Cellular and Biomolecular Analysis Report, NU, Singapore TNF-alpha ELISA Practical Report Aim of this experiment: culture U937 cells, differentiate them with PMA

TNF-alpha ELISA Practical Report Aim of this experiment: culture U937 cells, differentiate them with PMA, stimulate them with a range of LPS conc. to make them secrete TNF-alpha. Then, detect the amount of TNF-alpha secreted using ELISA.

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